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dc.rights.licenseCopyright 2020, Mary Ann Liebert, Inc., publishers-
dc.contributor.advisorUnidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737-
dc.contributor.authorMaggiori, Catherine-
dc.contributor.authorStromberg, J.-
dc.contributor.authorBlanco, Y.-
dc.contributor.authorGoordial, J.-
dc.contributor.authorCloutis, E.-
dc.contributor.authorGarcía Villadangos, M.-
dc.contributor.authorParro García, V.-
dc.contributor.authorWhyte, Lyle-
dc.identifier.citationAstrobiology 20(3): 375-393(2020)es
dc.description.abstractNo instrument capable of direct life detection has been included on a mission payload to Mars since NASA's Viking missions in the 1970s. This prevents us from discovering whether life is or ever was present on Mars. DNA is an ideal target biosignature since it is unambiguous, nonspecific, and readily detectable with nanopore sequencing. Here, we present a proof-of-concept utilization of the Oxford Nanopore Technologies (ONT) MinION sequencer for direct life detection and show how it can complement results from established space mission instruments. We used nanopore sequencing data from the MinION to detect and characterize the microbial life in a set of paleochannels near Hanksville, UT, with supporting data from X-ray diffraction, reflectance spectroscopy, Raman spectroscopy, and Life Detector Chip (LDChip) microarray immunoassay analyses. These paleochannels are analogs to martian sinuous ridges. The MinION-generated metagenomes reveal a rich microbial community dominated by bacteria and containing radioresistant, psychrophilic, and halophilic taxa. With spectral data and LDChip immunoassays, these metagenomes were linked to the surrounding Mars analog environment and potential metabolisms (e.g., methane production and perchlorate reduction). This shows a high degree of synergy between these techniques for detecting and characterizing biosignatures. We also resolved a prospective lower limit of ∼0.001 ng of DNA required for successful sequencing. This work represents the first determination of the MinION's DNA detection limits beyond ONT recommendations and the first whole metagenome analysis of a sinuous ridge
dc.description.sponsorshipThis work was funded by the Canadian Space Agency Flights and Fieldwork for the Advancement of Science and Technology (FAST) and Mars Sample Return (MSR) grants, the McGill Space Institute graduate student fellowship and postdoctoral fellowship, and the Spanish Ministry of Science project no. ESP2015-69540-R (MINECO/FEDER); With funding from the Spanish government through the "María de Maeztu Unit of Excellence" accreditation (MDM-2017-0737).es
dc.publisherMary Ann Liebert Publisherses
dc.subjectMinION sequencinges
dc.subjectLife detectiones
dc.subjectMars analoges
dc.titleThe Limits, Capabilities, and Potential for Life Detection with MinION Sequencing in a Paleochannel Mars Analoges
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO)-
dc.description.peerreviewedPeer reviewes
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