Copyright © Cambridge University Press 2011Sellek Cano, RicelaNiemcewicz, MarcinOlsen, JaranBassy Alvarez, OlgaLorenzo Lozano, P.Martí, L.Roszowiak, A.Kocik, J.Cabria Ramos, J. C.2026-07-162026-07-162012-08-05Epidemiology & Infection 140(8): 1389 - 13990950-2688https://www.cambridge.org/core/journals/epidemiology-and-infection/article/phenotypic-and-genetic-analyses-of-111-clinical-and-environmental-o1-o139-and-nono1o139-vibrio-cholerae-strains-from-different-geographical-areas/716E5C116CE1B5102F6D9EC1CAFC86D8https://hdl.handle.net/20.500.12666/1876A total of 111 clinical and environmental O1, O139 and non-O1/O139 Vibrio cholerae strains isolated between 1978 and 2008 from different geographical areas were typed using a combination of methods: antibiotic susceptibility, biochemical test, serogroup, serotype, biotype, sequences containing variable numbers of tandem repeats (VNTRs) and virulence genes ctxA and tcpA amplification. As a result of the performed typing work, the strains were organized into four clusters: cluster A1 included clinical O1 Ogawa and O139 serogroup strains (ctxA + and tcpA +); cluster A2 included clinical non-O1/O139 strains (ctxA − and tcpA −), as well as environmental O1 Inaba and non-O1/O139 strains (ctxA − and tcpA −/tcpA +); cluster B1 contained two clinical O1 strains and environmental non-O1/O139 strains (ctxA − and tcpA +/tcpA −); cluster B2 contained clinical O1 Inaba and Ogawa strains (ctxA + and tcpA +). The results of this work illustrate the advantage of combining several typing methods to discriminate between clinical and environmental V. cholerae strains.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Bacterial typinginfo:eu-repo/semantics/article10.1017/S09502688110021471469-4409info:eu-repo/semantics/openAccess