Examinando por Autor "Murillo, N."

Mostrando 1 - 2 de 2

Acceso Abierto
Biofunctionalization of nylon nanofibers to be used in immunobiosensor for biological warfare agents detecting
(Formatex Research Center, 2018) Peraile, Inés; Lorenzo Lozano, P.; Murillo, N.; Maudes, J.; Rozas Sanz, Gabriel; Pérez Márquez, Ana; González López, L.; Cabria Ramos, J. C.; Gil García, M.
The use of biological warfare agents involves a growing threat to society. Thus, the most countries have been forced to increase resources in research for their detection and identification. One of the critical points in the effective fight against these agents is the development of devices that allow their detection and early identification. The best choice is the immunobiosensors easy to use on-site. However, how the antibody is attached to the biosensor surface, in terms of density and orientation, will determine the diagnosis capability of the device. In this study, both a functional nanofiber able to increase the surface / volume relation, and a chemicallysimilar planar membrane were used as support for the immobilization of antibodies. Different antibody immobilization systems were carried out to biofunctionalize both surfaces: passive adsorption, covalent bond by glutaraldehyde and well-oriented immobilization by protein A/G. Our results showed that nanofibers in combination with protein A/G were a very effective immunocapture system for being used in a biosensor.
Acceso Abierto
Label-free optical biosensing using low-cost electrospun polymeric nanofibers
(MDPI Ag, 2020-12) Martínez Pérez, P.; Ponce Alcántara, S.; Murillo, Nieves; Pérez Márquez, Ana; Maudes, J.; Peraile, Inés; González López, L.; Gil García, M.; Lorenzo Lozano, P.; García Rupérez, J.; Generalitat Valenciana (GVA); Ministerio de Economía y Competitividad (MINECO); Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.