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URI permanente para esta colecciónhttps://digital.inta.es/handle/20.500.12666/83
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Examinando (Terrestre) Artículos por Autor "García Rupérez, J."
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Publicación Acceso Abierto Label-free optical biosensing using low-cost electrospun polymeric nanofibers(MDPI Ag, 2020-12) Martínez Pérez, P.; Ponce Alcántara, S.; Murillo, Nieves; Pérez Márquez, Ana; Maudes, J.; Peraile, Inés; González López, L.; Gil García, M.; Lorenzo Lozano, P.; García Rupérez, J.; Generalitat Valenciana (GVA); Ministerio de Economía y Competitividad (MINECO); Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.
